山形达也(Yamagata Tatsuya)

教授 博士生导师
[性 别]： 男
[国 籍]： 日本
[出生日期]： 1937年1月1日
[出 生 地]： 东京都目黑区

[学历]
1960年3月 东京大学理学部化学科毕业
1962年3月 东京大学大学院化学研究科生物化学理学硕士毕业
1968年2月 名古屋大学理学博士毕业

[简历]
1962年4月-1968年2月 名古屋大学理学部化学科助教
1968年3月-1973年4月 名古屋大学理学部化学科讲师
1969年8月-1971年10月 美国芝加哥大学医学部小儿科研究员
1973年5月-1973年10月 名古屋大学理学部化学科副教授
1973年11月-1982年1月 三菱化成生命科学研究所发生生物学研究室主任研究员
1982年2月-1987年3月 三菱化成生命科学研究所发展研究室室长
1987年4月-1993年3月 三菱化成生命科学研究所复合糖质研究室室长
1988年4月-1993年3月 兼任三菱化成生命科学研究所细胞识别研究部部长
1993年4月-1998年3月 东京工业大学生命理工学部客座教授
1998年4月-2003年3月 日本皮革研究所?糖锁情报工学部?理事研究员，教授
2001年6月-2003年3月 沈阳药科大学客座教授
2003年3月 沈阳药科大学教授，博士生导师

[获奖]
1969年 日本生化学会奖

[所属学会]

1961年- 日本生化学会 评议员
1985年- 日本糖质学会 名誉会员
1989年-1995年 FCCA (Forum: Carbohydrates Coming of Age） 创立者，干事长
1989年-1999年 Trends in Glycoscience and Glycotechnology 主编
1998年-2003年 东京糖锁研究会GlycoTOKYO 会长
1996年- Society for Glycobiology
1997年- American Soc. Biochem. & Mol. Biol.
1996年- 日本癌学会

[主要研究课题]：
1． 硫酸软骨素分解酶及硫酸软骨素的构造
2． 胚胎发生研究及蛋白聚糖M的发现
3． V型胶原蛋白的构造
4． 动物凝集素的作用
5． 糖脂分解酶的发现及应用
6． 糖脂机能的研究
7． 肿瘤的转移与糖脂的机能
8．利用糖链引物用细胞制备糖链

[研究方向]：
1． 虽然人们已经清楚肿瘤是由基因突变所引起的，但是目前采用基因治疗仍然很困难。为了防治癌症患者的死亡，本实验室致力于肿瘤转移机制的研究，尤其是对神经节苷脂抑制肿瘤转移性的细胞信号转导通路的研究，希望能够发现抑制肿瘤转移的药物。
2． 细胞表面的糖链与细胞的识别，粘附，移动，分裂有着密切的关系，为了研究糖链的机能，本实验室致力于利用细胞合成各种糖链的研究。我们认为如果能清楚糖链调控细胞功能的机理，不仅能够解释一些疾病的病因，而且还能开发出相应的治疗药物。

[主要论文]：（发表于1986年后）
1. Ito, M., and Yamagata, T.,
A novel glycosphingolipid-degrading enzyme cleaves the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids.
J. Biol. Chem., 261, 14278-14282 (1986)
2. Ito, M., Hirabayashi, Y., and Yamagata, T.,
Substrate specificity of endo-b-galactosidases from Flavobacterium keratolyticus and Escherichia freundii is different from that of Pseudomonas sp.
J. Biochem., 100, 773-780 (1986)
3. Ohara, T., and Yamagata, T.,
Isolation and characterization of galactose-binding proteins from new-born mice.
Biochim. Biophys. Acta, 884, 344-354 (1986)
4. Yamagata, S., and Yamagata, Y.,
The staining of type V collagen obtained from FBJ virus-induced osteosarcoma with concanavalin A.
Biochem. Biophy. Res. Commun., 136, 857-862 (1986)
5. Higashi, H., Hirabayashi, Y., Ito, M., Yamagata, T., Matsumoto, M., Ueda, S., and Kato S.,
Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase.
J. Biochem., 102, 291-296 (1987)
6. Hirabayashi, J., Oda, Y., Ohara, T., Yamagata, T., and Kasai, K.,
Immunological cross-reactivity between β-galactoside-binding lectins of vertebrates. Possible relationship of antigenecity to oligomeric structure.
Biochim. Biophys. Acta, 916, 321-327 (1987)
7. Mikawa, T., Sashida, R., Ito, M., and Yamagata, T.,
Rhodocuccus sp. producing endoglycoceramidase.
Mitsubishi Chemical R & D Review, 1, 34-39 (1987)(in Japanese)
8. Shimamura, M., Hayase, T., Ito, M., Rasilo, M.-L., and Yamagata, T.,
Characterization of a major neutral glycolipid in PC12 cells as III3galα-globotriaosylceramide by the method for determing glycosphingolipid saccharide sequence with endoglycoceramidase.
J. Biol. Chem., 263, 12124-12128 (1988)
9. Rasilo M.-L., and Yamagata, T.,
Occurrence of glucose polymer in undifferentiated PC12 cells.
FEBS letters, 227, 197-194 (1988)
10. Rasilo M.-L., and Yamagata, T.,
Characterization of a glucose polymer from PC12 cells and neuronal cells of rat embryo.
J. Biochem., 104, 742-754 (1988)
11. Ito, M., and Yamagata, T.,
Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp.
J. Biol. Chem., 264, 9510-9519 (1989)
12. Fukaya, N., Ito, M., Iwata, H., and Yamagata, T.,
Characterization of the glycosphingolipids of pig cortical bone and cartilage.
Biochim. Biophys. Acta, 1004, 108-116 (1989)
13. Rasilo, M.-L., Ito, M., and Yamagata, T.,
Liberation of oligosaccharides from glycosphingolipids on PC12 cell surface with endoglycoceramidase.
Biochem. Biophys. Res. Commun., 162, 1093-1099 (1989)
14. Rasilo, M.-L., and Yamagata, T.,
The glucose polymer of PC12 cells is susceptible to trypsinization.
Biochem. Biophys. Res. Commun., 159, 1-6 (1989)
15. Higashi, H., Ito, M., Fukaya, N., Yamagata, S., and Yamagata, T.,
Two-dimensional mapping by the high-performance liquid chromatography of oligosaccharides released from glycosphingolipids by endoglycoceramidase.
Anal. Biochem., 186, 355-362 (1990)
16. Kubo, T., Hayase, T., Morimoto, Y., Kondo, J., Matsumoto, R., Nagai, K., and Yamagata, T.,
Structural analysis of the carbohydrate chain of recombinant apolipoprotein E.
Mitsubihsi Kasei R & D Review, 4, 1-7 (1990)(in Japanese)
17. Ito, M., Ikegami, Y., and Yamagata, T.,
Activator proteins for glycosphingolipid hydrolysis by endoglycoceramidases.
J. Biol. Chem., 266, 7919-7926 (1991)
18. Shimamura, M., Oku, M., and Yamagata, T.,
The retarded rate of acid-catalyzed solvolysis of glycoside bonds between reducing- end glycose residue and ceramide in glycosphingolipids compared with that of glycoside bonds between hexopyranosides.
FEBS letters, 290, 213-215 (1991)
19. Ito, M., Ikegami, Y., Omori, A., and Yamagata, T.,
Conversion of endoglycoceramidase-activator II by trypsin to the 27.9 kDa polypeptide possessing full activity: Purification of activator for endoglycoceramidase by trypsin treatment followed by trypsin-inhibitor agarose column application.
J. Biochem. 110, 328-332 (1991)
20. Yamagata, T., and Yamazaki, K.,
Implanting mouse embryo stain with a LNF-I bearing fluorescent probe at their mural trophectodermal side.
Biochem. Biophys. Res. Commun., 181, 1004-1009 (1991)
21. Higashi, H., Omori, A., and Yamagata, T.
Calmodulin: A ganglioside-binding protein.
J. Biol. Chem., 267, 9831-9838 (1992)
22. Higashi, H., and Yamagata, T.,
Mechanism for ganglioside-mediated modulation of calmodulin-dependent enzyme.
J. Biol. Chem., 267, 9839-9843 (1992)
23. Shimamura, M., Oku, M., and Yamagata, T.,
Presence of murine fetal liver cells capable of being induced to differentiate in vitro into T cell receptor αβ positive cells.
Eur. J. Immunol., 22, 2159-2164 (1992)
24. Shimamura, M., Oku, M., Ohta, S., and Yamagata, T.,
Hematopoietic cell lines capable of colonizing the thymus following in vivo transfer expressed T cell receptor δ gene immature mRNA.
Immunol. 77, 369-376 (1992)
25. Shimamura, M., Oku, M., and Yamagata, T.,
A novel ganglioside expressed by mouse hematopoietic cell lines.
J. Biol. Chem., 267, 18810-18813 (1992)
26. Higashi, H., and Yamagata, T.,
Full activation without calmodulin of calmodulin-dependent cyclic nucleotide phosphodiesterase by acidic glycosphingolipids; GM3, sialosylneolactotetraosylceramide and sulfatide.
FEBS letters, 314, 53-57 (1992)
27. Ito, M., Ikegami, Y., and Yamagata, T.,
Kinetics of endoglycoceramidase action toward cell-surface glycosphingolipids of erythrocytes.
Eur. J. Biochem., 218, 645-649 (1993)
28. Ito, M., Ikegami, Y., Tai, T., and Yamagata, T.,
Specific hydrolysis of intact erythrocyte cell-surface glycosphingolipids by endoglycoceramidase.
Eur. J. Biochem., 218, 637-643 (1993)
29. Ponce, R.H., Yanagimachi, R., Urch, U.A., Yamagata, T., and Ito, M.,
Retention of hamster oolema fusibility with spermatozoa after various enzyme treatments: A search for the molecules involved in sperm-egg fusion.
Zygotes, 1, 163-171 (1993)
30. Shimamura, M., Shibuya, N., Ito, M., and Yamagata, T.,
Repulsive contribution of surface sialic acid residues to cell adhesion to substratum.
Biochem. Mol. Biol. Internatl., 33, 871-878 (1994)
31. Muramoto, K., Kawahara, M., Kobayashi, K., Ito, M., Yamagata, T., and Koroda, Y.,
Endoglycoceramidase treatment inhibits synchorous oscillations of intercellular Ca2+ in cultured cortical neuron.
Biochem. Biophys. Res. Commun., 202, 398-402 (1994)
32. Li. J., Ito, M., Zhang, G., and Yamagata, T.,
The hydrolysis of cell surface glycoshingolipids by endoglycoceramidase reduces epidermal growth factor receptor phosphorylation in A431 cells.
Glycobiology, 5, 343-350 (1995)
33. Nagatsuka, Y., Watari, S., Yasuda, T., Higashi, H., Yamagata, T., and Ono, Y.,
Production of human monoclonal antibodies to i blood group by EBV-induced transformation: Possible presence of a new glycolipid in cord blood cell membranes and human hematopoietic cell lines.
Immunol. Lett., 46, 93-100 (1995)
34. Miura, R.O., Yamagata, S., Harada, T., Miura, Y., and Yamagata, T.,
Analysis of glycosaminoglycan degrading enzymes by substrate gel electrophoresis (Zymography).
Anal. Biochem., 225, 333-340 (1995)
35. Miura, Y., Arai, T., and Yamagata, T.,
Synthesis of amphiphilic lactosides that possess a lactosylceramide-mimicking N-acyl structure.
Carbohydrate Res., 289, 193-199 (1996)
36. Higashi, H., Yoshida, S., Sato, K., and Yamagata, T.,
Interaction of ganglioside and specific peptide sequences as a mechanism for modulation of calmodulin-dependent enzymes by gangliosides.
J. Biochem., 120, 66-73 (1996)
37. Nagatsuka, Y., Yamaki, M., Watarai, S., Yasuda, T., Higashi, H., Yamagata, T., and Ono, Y.,
The Epstein-Barr virus (EBV) alters the B cell glycolipid which is recognized by the human monoclonal antibody to i-blood antigen.
Virus Research, 43, 57-68 (1996)
38. Hyuga, S., Yamagata, S., Tai, T., and Yamagata, T.,
Inhibition of highly metastatic FBJ-LL cell migration by ganglioside GD1a highly expressed in poorly metastatic FBJ-S1 cells.
Biochem. Biophys. Res. Commun., 231, 340-343 (1997)
39. Kikkawa, F., Tamakoshi, K., Nawa, A., Shibata, K., Yamagata, S., Yamagata, T., and Suganuma, N.,
Positive correlation between inhibitors of matrix metalloproteinase 1 and matrix metalloproteinases in malignant ovarian tumor tissues.
J Cancer Lett., 120, 109-115 (1997)
40. Tamakoshi, K., Kikkawa, F., Yamagata, S., Yamagata, T., and Tomoda, Y.,
Hyaluronidase activity in gynecologic cancer tissue with different metastatic forms.
British J. Cancer, 75, 1807-1811 (1997)
41. Suzuki, T., Horie, G., Yamazaki, Y., Kawabe, K., Masiuda, H., Miyamoto, D., Matsuda, M., Nishimura, S.,Yamagata, T., Ito, T., Kida, H., Kawaoka, Y., and Suzuki, T.,
Swine influenza virus strains recongnize sialylsugar chains containing the molecular species of sialic acid predominatly present in the swine tracheal epithelium.
42. Miura, Y., and Yamagata, T.,
Glycosylation of lactosylceramide analogs in animal cells; Amphipathic disaccharide primers for glycosphingolipid synthesis.
43. Podyma, K.A., Yamagata, S., Sakata, K., and Yamagata, T.,
Difference of hyaluronidase produced by human tumor cell lines with hyaluronidase present in human serum.as revealed by zymography
Biochem. Biophys. Res. Commun., 241, 446-450 (1997)
44. Nakajima, H., Miura, Y., and Yamagata, T.,
Glycosylation of amphipathic lactoside primers with consequent inhibition of endogenous glysosphingolipid synthesis.
J. Biochem., 124, 148-156 (1998)
45. Ji, L., Ito, M., Zhang, G., Hirabayashi, Y., Inokuchi, J., and Yamagata , T.,
Effects of endoglycoceramidase of D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol on glucose uptake, glycolysis, and mitochondrial respiration in HL60 cells.
Arch Biochem. Biophys., 359, 107-14 (1998)
46. Miura, Y., Arai, T., Ohtake, A., Ito, M., Yamamoto, K., and Yamagata, T.,
Requirement for a different hydrophobic moiety and reliable chromogenic substrate for endo-type glycosylceramidases
Glycobiology, 9, 957-960 (1999)
47. Nagaya, N., Yamagata, T., Yamagata, S., Iyoda, K., Ito, H., Hasegawa, Y., and Iwata, H.,
Examination of synovial fluid and serum hyaluronidase activity as a joint marker in rheumatoid arthritis and osteoarthritis patients (by zymography)
Ann. Rheum. Dis., 58, 186-188 (1999)
48. Hyuga, S., Yamagata, S., Takatsu, Y., Hyuga, M., Nakanishi, H., Furukawa, K. and Yamagata, T.,
Suppression of FBJ-LL cell adhesion to vitronectin by ganglioside GD1a and loss of metastatic capacity.
International J. Cancer, 83, 685-691 (1999)
49. Hyuga,S., Kawasaki, N., Hashimoto, O., Hyuga, M., Ohta, M., Yamagata, S., Yamagata, T., and Hayakawa, T.,
Possible role of hepatosyte growth gactor/scatter factor and activin A produced by the target organ in liver metastasis.
Cancer Lett., 153, 137-143 (2000)
50 Kasuya, Maria C. Z., Wang, L. X., Lee, Y. C, Mitsuki, M., Nakajima, H.,Miura, Y., Sato, T., Hatanaka, K., Yamagata, S., and Yamagata, T.,
Azido glycoside primer: A versatile building block for the biocombinatorial synthesis of glycosphingolipid analogues.
Carbohydr. Res. 329, 755-763 (2000)
51. Hyuga, S., Kawasaki, N., Ohta, M., Shibayama, R., Kawanishi, T., Yamagata, S., Yamagata, T., and Hayakawa, T.,
Ganglioside GD1a inhibits the HGF/SF-induced motility and scattering of cancer cells through suppression of tyrosine-phosphorylation of c-Met.
International J. Cancer, 94, 328-334 (2001)
52. Nadanaka, S., Sato, C., Kitajima, K., Katagiri, K., Irie, S., and Yamagata, T.,
Occurrence of Oligosialic Acids on Integrin alpha5 Subunit and Their Involvement in Cell Adhesion to Fibronectin.
J. Biol. Chem. 276, 33657-33664 (2001)

[主要著作]（１９９０年以后）
１. 糖鎖の検出 (電流測定法: アンペロメトリィ)
新生化学実験講座3, 糖質I, 糖タンパク質, (化学同人) 149-156 (1990)
2. エンドグリコセラミダーゼ
新生化学実験講座4, 脂質III, 糖脂質, (化学同人) 404-408 (1990)
3. 着床における糖鎖の役割
蛋白質?核酸?酵素, 37, 1783-1788 (1992)
4. 糖脂質糖鎖の細胞工学
糖鎖工学（産業調査会編）, 204-214 (1992)
5. 細胞質内にもある糖脂質
細胞, 25, 105-110 (1993)
6. 糖脂質の運命
グリコバイオロジ?シリ?ズ第２巻, (講談社), 179-191 (1993)
7. 細胞の恒常性維持と糖鎖
”糖鎖”第Ｉ巻, 「糖鎖と生命」（永井克孝ほか編）, (東京化学同人), pp.78-85（1994）
8. ガングリオシドとカルモジュリンの結合とガングリオシドによるカルモジュリン依存性酵素の活性調節
ガングリオシド研究法２, (学会出版センター), pp.180-188 (1995)
9. ザイモグラフィーによるグリコサミノグリカン分解酵素の研究
週刊「医学のあゆみ?細胞外マトリックス」、177、36-41、(1996)
10. ヒアルロニダーゼのザイモグラフィー(Zymography)

[联系方式]
Tatsuya Yamagata, D.Sc.
Professor of Biochemistry, Cell Biology and Glycobiology
Shenyang Pharmaceutical University
P. O. Box 29
103 WenHua Road
Shenyang 110016
P. R. China
Business phone: +86-24-2384-3711-3005
Home phone: +86-24-2390-2575
Cellular phone: 13332468824
E-mail: tcyamagata@pop16.odn.ne.jp（日文或英文）
主页地址：http://niccou.51.net/